Enzyme Retrieval Solution (Ready-to-Use)

Catalog No.
MYF11
Specifications
3 ml (MYF11-1)

Product Details

For antigen retrieval prior to immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections, to enhance staining performance in IHC assays.
    1. Soak tissue sections in two changes of fresh xylene for 10 min each, then in absolute ethanol, 95% ethanol, and 85% ethanol for 5 min each. Rinse gently in distilled water for 1 min.
    2. Blot excess liquid around the tissue; circle the tissue edge 2–3 mm away with an IHC pen. Rinse gently in distilled water twice for 3 min each, then immerse in PBS buffer.
    3. Apply sufficient enzyme retrieval solution to cover the tissue completely. Incubate at 37°C in a humid chamber for 10 min. Wash with PBS 2–3 times and rinse gently twice for 3 min each.
    4. Incubate sections in peroxidase blocking reagent (3% H2O2) for 10 min. Rinse gently in distilled water twice for 3 min each, then immerse in PBS buffer.
    5. Apply sufficient primary antibody working solution to cover the tissue completely. Incubate at room temperature in a humid chamber for 30–60 min. Wash with PBS 2–3 times and rinse gently twice for 3 min each.
    6. Apply sufficient secondary antibody working solution to cover the tissue completely. Incubate at room temperature in a humid chamber for 20 min. Wash with PBS 2–3 times and rinse gently twice for 3 min each.
    7. Apply sufficient DAB working solution to cover the tissue completely. Incubate at room temperature in a humid chamber for 5 min. Stop color development with distilled water.
    8. Counterstain in hematoxylin for 8–10 s. Rinse thoroughly with distilled water. Bluing in 1% lithium carbonate for 8–15 s, then rinse with distilled water.
    9. Dehydrate through 85% ethanol, 95% ethanol, and absolute ethanol for 3 min each, then in one change of fresh xylene for 5–10 min. Mount with neutral resin and examine under a microscope.
    Application
    IHC-P
    Form
    Liquid
    Shipping
    Biological refrigerated ice packs
    Storage conditions
    2–8°C
    Shelf life
    1 year
    Storage notes
    Store protected from light and heat