After-sales review for staining issues and experimental troubleshooting.
After you submit an after-sales request, we will provide an Antibody Performance Feedback Form. Please complete:
• Product lot number and dilution
• Experimental parameters (antigen retrieval, antibody incubation, DAB developing time, etc.)
• Photos of staining results
Within 7 business days of receiving your application, we will provide in-house test results and recommended conditions. Paraffin tissue sections used in testing can be provided on request.
First verify antigen retrieval, primary dilution, and secondary/detection compatibility. We can also provide photos from our QC staining. If signal is still absent after ruling out variables and handling issues, you may apply for after-sales review after submitting your results.
Provide side-by-side staining from old and new lots with detailed records. Within 7 business days we will share in-house test results and recommended conditions, and return the paraffin sections used in testing. We can also run testing under your conditions and provide results on request.
Try reducing retrieval intensity, increasing antibody dilution, shortening incubation, and adding wash steps. If results do not improve after several optimizations, provide full staining photos and the paraffin sections used. Within 7 business days we will provide in-house test results and recommended conditions, and return the paraffin sections used in testing.
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