1. Antibody concentration too low or incubation time insufficient. 2. Incomplete antigen retrieval. 3. Over-fixation of tissue. 4. Insufficient detection system sensitivity. 5. Inconsistent section thickness. Solutions: optimize working concentration and extend incubation; ensure fresh retrieval buffer and proper temperature; control fixation time; use a high-sensitivity detection system; keep section thickness uniform.
Products
What you need to know about antibody selection and product use.
Consider assay type (WB, IHC, IF, etc.), sample species, antibody specificity, and clonality (monoclonal/polyclonal). Please refer to our product catalog or contact technical support.
You can download datasheets directly from the product detail page, or search by catalog number in the Product Center.