Enzyme Retrieval Solution (Ready-to-use) Product Manual

Enzymatic Retrieval Solution(ready-to-use with type)

Instruction Manual

        Manual No.:MYF11253

[Product Name]

Generic Name:Enzymatic Retrieval Solution(ready-to-use with type)

[Packaging Specifications]

3ml/box

[Intended Use]

with for formalin-fixed , paraffin-embedded tissue sectionsimmunohistochemicalbefore staining Antigen Retrieval,enhance immunohistochemicalexperiment staining results.

Test Summary and Description

During tissue processing, chemical reagents mask antigens and heat distorts some antigen peptide chains, preventing display during IHC staining. Antigen retrieval uses chemical and heat treatment to re-expose or restore these antigens.Citrate, EDTAor Tris etc. buffers in heat conditions can unmask formalin-blocked antigens re-exposed ,without damaging antigen epitopes .thereby improving antigen detection rate, ,reducing background staining, ,improving diagnostic accuracy.

in heat-induced Antigen Retrievalbefore the advent of ,Trypsin, proteinenzyme KPepsin enzymes induced epitope retrieval was a common with method.Pepsin antigen retrieval solution exposes antigen epitopes in paraffin sections, greatly improving immunostaining.Paraffin sections usually require antigen retrieval; frozen sections may not. Antigen retrieval improves paraffin section staining and can also improve frozen section staining. Consider retrieval when frozen section results are unsatisfactory.

[Main Components]

proteinenzyme K

[Storage Conditions and Validity Period]

Kit 2~8℃storage.with validity period 12 months.

[Operating Procedures]

1(1) Paraffin Sections

1, Deparaffinize to water

①Xylene3 times,each 3~5min.

②Anhydrous ethanoldehydration2 times,each 3~5min.

③95% ethanol,3~5min.

④90% ethanol,3~5min.                              

⑤80% ethanol,3~5min.                            

⑥70% ethanol,3~5min.                             

⑦Rinse with distilled water2 times,each 3~5min.                      

2, Antigen Retrieval:Apply pepsin antigen retrieval solution drops on the slide ,37℃incubation10~25min.

3, Wash with IHC wash buffer or tap water1~2 times,each 3~5min,rinse thoroughly.

4, proceed with subsequent IHC steps.

(2) Frozen Sections: 

1, Wash sections with IHC wash buffer or tap water5min.

2, Antigen Retrieval:Immerse sections in pepsin antigen retrieval solution,37℃incubation10~25min.

3, Wash with IHC wash buffer or tap water1~2 times,each 3~5min,rinse thoroughly.

4, proceed with subsequent IHC steps.

(3) Other Samples:

Process other samples as paraffin or frozen sections.

[Precautions]

pepsin proteinBring enzymatic retrieval solution to room temperature before use; avoid repeated freeze-thaw cycles.

Immerse in pepsin antigen retrieval solution,Optimize incubation time according to sample type and purposepurpose.

For your safety, wear a lab coat and disposable gloves.